B-RAF is a protein found in the BRAF human gene. In many cases, it is referred to as a proto-oncogene. The inhibitor is usually utilized in studies that focus on gaining information about melanoma tumors or curing them. It is used by researchers everywhere to stop the cancerous growth in animals and humans. Many studies on skin cancer have shown that mutations in this gene are present in those with melanoma tumors. Using this substance could increase your patient’s survival if they have such cancer. You may also be able to research other tumors, such as ovarian cancer, colorectal carcinoma, and papillary thyroid carcinoma.
More Information
The BRAF inhibitor does have a clone called pBR1, and the immunogen is the full-length recombinant human protein of the same name. The isotype is the Rat IgG2a, and it has an undetermined epitope with a molecular weight of 95 kDa.
Applications
This antibody is designed to be used with Immunohistochemistry applications. It can also be used for Western Blotting, which is recommended but untested.
The IHC procedure includes using formalin-fixed or paraffin-embedded tissues, as well as deparaffinized slides.
You’ll find a concentrated formula and a pre-diluted version. When using the concentrate, you will need to dilute the antibody using a ratio of 1:100 in an antibody Diluent. This is an estimated dilution rate, and you may need to make changes based on your protocol.
To retrieve the antigen, you should boil the tissue section using an EDTA buffer with a pH of 8.0. Do this for ten minutes and let it cool for 20 minutes, and then incubate for 30 minutes.
The positive control is the colon carcinoma with cellular localization occurring in the cytoplasm.
The BRAF inhibitor can help you research skin cancer and other cancers. Visit Spring Bioscience at to learn more today.
