The FOXP3 antibody is a member of the forkhead/winged-helix family in the transcriptional regulator area and is also a 432 amino acid protein. It is crucial for normal immune homeostasis and is expressed at high levels in CD35 + CD4 positive T-cells and low levels in CD4 positive/CD35 negative cells. It is not seen in CD4 negative-CD8 positive T-cells at all. It could be one of the few master regulatory genes and an excellent market for regulatory T-cells.
More Information
The FOXP3 antibody has no known clone, and the immunogen is located in the synthetic peptide derived from the human protein of the same name in the c-terminus. The isotype is the Rabbit IgG, and it has no known epitope. It also has a molecular weight of 50kDa.
Applications
This antibody uses the Immunohistochemistry application and requires a paraffin-embedded or Formalin-fixed tissue sample. Deparaffinized slides should be used at all times and can be cleaned with xylene and its alternatives.
You’ll find a concentrated version of this product and should dilute the antibody using a ratio of 1:200. However, dilutions are estimates, and yours may be different. If your protocols state this ratio, you can also use the pre-diluted formula available for purchase.
To retrieve the antigen, you will need to boil your tissue section for 10 minutes in a 1mM EDTA with a pH of 8.0 for ten minutes. Allow it to cool for 20 minutes to reach room temperature. Incubation periods are 30 minutes at room temperature, as well.
The positive control is the tonsil with cellular localization occurring in the nucleus. You’ll find a variety of sizes available, allowing you to purchase only what you need.
The FOXP3 antibody is an excellent product to use for a variety of research needs. Visit Spring Bioscience to learn more about purchasing options.
