The CD44 is a cell-surface glycoprotein that’s expressed on monocytes, granulocytes, and lymphocytes. It is also considered a homing cell molecule (H-CAM), as well as a Phagocytic glycoprotein. It has been implicated in lymphocyte homing, tumor metastasis and cell migration.
This antibody is intended for research use only and has a clone called SPM 521. The immunogen is the synthetic peptide that corresponds to the human CD44. The isotype is the IgG 2a, and it has no determined epitope. The molecular weight of the CD44 antibody is 80kDa.
Applications
The CD44 antibody is designed to be used with Western Blotting and Immunohistochemistry.
For Western Blotting, it is recommended to dilute the antibody using a ratio of 1:25, and it is not recommended to use a pre-diluted formulation. Incubate it for one hour at room temperature. The positive control is the HeLa cell lysate with a cellular localization in the membrane.
For Immunohistochemistry applications, you should use paraffin-embedded or Formalin-fixed tissues. Deparaffinized slides are required and can be achieved with xylene or its alternative, as well as graded alcohols.
You should dilute the antibody using a ratio of 1:50 unless you use the pre-diluted formula. Boil your tissue section in a 10mM citrate buffer with a pH of 6.0 for 10 minutes and allow to cool to room temperature for 20 minutes to retrieve the antigen. Incubate it for 30 minutes more at room temperature.
The positive control for IHC testing is the esophageal carcinoma with cellular localization in the membrane.
Options
You’ll find pre-diluted options that work for IHC applications only, as well as a 0.1ml, 0.5ml, and 1.0ml version.
The CD44 antibody should be tested when you are concerned that something is wrong with cell migration and other situations. Visit Spring Bioscience to learn more about their products and options.
